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recombinant human mouse rat activin a  (R&D Systems)


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    R&D Systems recombinant human mouse rat activin a
    Recombinant Human Mouse Rat Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 589 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+activin+a/Recombinant+Human%2FMouse%2FRat+Activin+A+Protein/pmc13120857-25-22-27
    Average 96 stars, based on 589 article reviews
    recombinant human mouse rat activin a - by Bioz Stars, 2026-09
    96/100 stars

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    Recombinant:

    Article Title: Chronic cerebral hypoperfusion induces venous dysfunction via EPAS1 regulation in mice.
    Article Snippet: To seed for endothelial differentiation, H9 were dissociated with accutase (Gibco, A1110501) into single cells, then plated onto Matrigel-coated plates at a density of 50,000 cells/cm2 in mTeSR1 medium supplemented with 5 μMROCK inhibitor, Y-27632 (STEMCELL Technologies, 72304). .. 24 h later, H9 cells were induced towards the mid-primitive streak differentiation pathway with 40 ng/ml recombinant human BMP-4 (R&D Systems, 314-BP/CF), 6 μM CHIR99021 (Sigma-Aldrich, SML1046), 4 μg/ml recombinant human FGF2 (R&D Systems, 233-FB/CF), PIK-90 (SigmaAldrich, 528117), recombinant human activin A (R&D Systems, 338- AC/CF) in Chemically Defined Medium (CDM) made up of 50% Ham’s F12 (Gibco, 31765092), 50% Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco, 31980097), 1mg/ml polyvinyl alcohol (SigmaAldrich, P8136), 1% chemically defined lipid concentrate (Gibco, 11905031), 450μM 1-thioglycerol (Sigma-Aldrich, M6145), 15μg/ml transferrin (Roche, 10652202001) and 0.7μg/ml recombinant human insulin (Roche, 11376497001). .. After 24 h, mid-primitive streak cells were differentiated into dorsal lateral mesoderm in CDM, supplemented with 40 ng/mL BMP4, 2.5μM GDC-0941 (STEMCELL Technologies, 73152), 10 μM Forskolin (Tocris, 1099), 2 μM SB505124 (Sigma-Aldrich, S4696), 100 ng/ml VEGF (R&D Systems, 293-VE/CF), 1 μM XAV939 (Tocris, 3748) and 200μg/mL L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich, A8960) Nature Communications | (2025) 16:6302 14 for 24 h. Following that, the dorsal lateral mesoderm was further differentiated towards either arterial endothelial induction or prevein induction, with a duration of 24 h. For arterial induction, CDM supplemented with 15 ng/mL recombinant human activin A, 250 nM DMH-1 (Tocris, 4126), 2.5 μM GDC-0941, 100ng/mL VEGF, 1 μM XAV939 and 200μg/ml L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate was used.

    Article Title: PAVR: High-Resolution Cellular Imaging via a Physics-Aware Volumetric Reconstruction Framework
    Article Snippet: MR90-IV (WiCell Research Institute) was maintained in mTeSR1 medium (Stem Cell Technologies) and induced for cardiac differentiation as previously described . .. Briefly, hiPSCs were treated with 100 μg/mL recombinant human activin A (R&D Systems) in insulin-free RPMI medium containing 2% B27 (RPMI-B27) on day 0. .. After 24 h, the medium was replaced with 10 μg/mL recombinant human bone morphogenic protein-4 (BMP4, R&D Systems) in RPMI-B27 insulin-free medium from day 1 to day 4.

    Article Title: Method for producing pluripotent stem cells
    Article Snippet: On day 1 of the culture, medium exchange was performed with a MEF-conditioned medium containing bFGF at a final concentration of 20 ng/mL. (Step 2-A: Induction of Differentiation into Ectoderm Cells) For the cells prepared in Step 1 of Example 7, medium exchange was performed on day 2 of the culture with KnockOut-DMEM (Thermo Fisher Scientific K.K.) containing knockout serum replacement (Thermo Fisher Scientific K.K.) at a final concentration of 10%, Noggin (R&D Systems, Inc.) at a final concentration of 500 ng/mL, and SB431542 (Tocris Bioscience) at a final concentration of 10 μM. .. Medium exchange was performed using the same medium on day 3 of the culture, and thereby an ectoderm cell population could be obtained on day 4 of the culture. (Step 2-B: Induction of Differentiation into Mesoderm Cells) For the cells prepared in Step 1 of Example 7, medium exchange was performed on day 2 of the culture with an RPMI-B27 (Thermo Fisher Scientific K.K.) medium to which recombinant human Activin A (R&D Systems, Inc.) was added at a final concentration of 100 ng/mL. .. Medium exchange was performed on days 3, 4, and 5 of the culture with an RPMI-B27 (Thermo Fisher Scientific K.K.) medium to which recombinant human BMP4 (R&D Systems, Inc.) was added at a final concentration of 10 ng/mL, and thereby a mesoderm cell population could be obtained on day 6 of the culture. (Step 2-C: Induction of Differentiation into Endoderm Cells) For the cells prepared in Step 1 of Example 7, medium exchange was performed on day 2 of the culture with an MCDB-131 medium to which NaHCO3 at a final concentration of 2.5 g/L, Glutamax (Thermo Fisher Scientific K.K.) at a final concentration of 1%, glucose at a final concentration of 5.5 mM, FAF-BSA (Proliant Inc. and Lampire Biological Laboratories, Inc.) at a final concentration of 0.1%, ITS: X (Thermo Fisher Scientific K.K.) diluted 50000-fold, WNT3A (R&D Systems, Inc.) at a final concentration of 20 ng/mL, and Activin A at a final concentration of 100 ng/mL were added.

    Article Title: HYPER-Net: Physics-Conditioned Self-Supervised Reconstruction for Fourier Light-Field Microscopy
    Article Snippet: MR90-IV (WiCell Research Institute) was maintained in mTeSR1 medium (Stem Cell Technologies) and induced for cardiac differentiation as previously described . .. Briefly, hiPSCs were treated with 100 ng/ml recombinant human activin A (R&D Systems) in RPMI medium with 2% B27 insulin-free (RPMI/B27 insulin-free medium) on day 0. .. After 24 h, the medium was replaced with 10 ng/ml recombinant human bone morphogenetic protein-4 (BMP4, R&D Systems) in RPMI/B27 insulin-free medium, as used from days 1 to 4.

    Article Title: Mechano-activated chondroprogenitors (MACs) drive intrinsic cartilage repair; a process that is arrested in osteoarthritis
    Article Snippet: .. Human recombinant FGF2 was purchased from Peprotech EC Ltd. Recombinant human Activin A (MAB3381), TGF-βR3 (QK054), FGF2 neutralizing antibody (AF-233-NA) and Activin A neutralizing antibody (MAB3381) were obtained from R&D Systems. ..

    Article Title: Chronic cerebral hypoperfusion induces venous dysfunction via EPAS1 regulation in mice
    Article Snippet: To seed for endothelial differentiation, H9 were dissociated with accutase (Gibco, A1110501) into single cells, then plated onto Matrigel-coated plates at a density of 50,000 cells/cm 2 in mTeSR1 medium supplemented with 5 μM ROCK inhibitor, Y-27632 (STEMCELL Technologies, 72304). .. 24 h later, H9 cells were induced towards the mid-primitive streak differentiation pathway with 40 ng/ml recombinant human BMP-4 (R&D Systems, 314-BP/CF), 6 μM CHIR99021 (Sigma-Aldrich, SML1046), 4 μg/ml recombinant human FGF2 (R&D Systems, 233-FB/CF), PIK-90 (Sigma-Aldrich, 528117), recombinant human activin A (R&D Systems, 338-AC/CF) in Chemically Defined Medium (CDM) made up of 50% Ham’s F12 (Gibco, 31765092), 50% Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco, 31980097), 1 mg/ml polyvinyl alcohol (Sigma-Aldrich, P8136), 1% chemically defined lipid concentrate (Gibco, 11905031), 450 μM 1-thioglycerol (Sigma-Aldrich, M6145), 15 μg/ml transferrin (Roche, 10652202001) and 0.7 μg/ml recombinant human insulin (Roche, 11376497001). .. After 24 h, mid-primitive streak cells were differentiated into dorsal lateral mesoderm in CDM, supplemented with 40 ng/mL BMP4, 2.5 μM GDC-0941 (STEMCELL Technologies, 73152), 10 μM Forskolin (Tocris, 1099), 2 μM SB505124 (Sigma-Aldrich, S4696), 100 ng/ml VEGF (R&D Systems, 293-VE/CF), 1 μM XAV939 (Tocris, 3748) and 200 μg/mL L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich, A8960) for 24 h. Following that, the dorsal lateral mesoderm was further differentiated towards either arterial endothelial induction or pre-vein induction, with a duration of 24 h. For arterial induction, CDM supplemented with 15 ng/mL recombinant human activin A, 250 nM DMH-1 (Tocris, 4126), 2.5 μM GDC-0941, 100 ng/mL VEGF, 1 μM XAV939 and 200 μg/ml L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate was used.

    Article Title: Universal donor cells and related methods
    Article Snippet: .. : hESC aggregates; XF HA: DMEM/F12 containing GlutaMAX, supplemented with 10% v/v of Xeno-free KnockOut Serum Replacement, 1% v/v non-essential amino acids, 1% v/v penicillin/streptomycin (all from Life Technologies), 10 ng/mL heregulin-1β (Peprotech) and 10 ng/mL activin A (R&D Systems); SP: StemPro ® hESC SFM (Life Technologies); r0.2FBS: RPMI 1640 (Mediatech); 0.2% FBS (HyClone), 1x GlutaMAX-1 (Life Technologies), 1% v/v penicillin/streptomycin; ITS: Insulin-Transferrin-Selenium (Life Technologies) diluted 1:5000 or 1:1000; A100: 100 ng/mL recombinant human Activin A (R&D Systems); W50: 50 ng/mL recombinant mouse Wnt3A (R&D Systems); K25: 25 ng/mL recombinant human KGF (R&D Systems); IV: 2.5 μM TGF-β RI Kinase inhibitor IV (EMD Bioscience); db: DMEM HI Glucose (HyClone) supplemented with 0.5x B-27 Supplement (Life Technologies), 1x GlutaMAX, and 1% v/v penicillin/streptomycin; CTT3: 0.25 μM KAAD-Cyclopamine (Toronto Research Chemicals) and 3 nM TTNPB (Sigma-Aldrich); N50: 50 ng/mL recombinant human Noggin (R&D Systems); K50: 50 ng/mL recombinant human KGF (R&D Systems); E50: 50 ng/mL recombinant human EGF (R&D Systems). ..

    Article Title: Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin
    Article Snippet: .. CDM was prepared with Neurobasal medium (Gibco, 21103-049) and DMEM high glucose (Sigma, D6429), supplemented with N2 supplement (1×; Gibco, 17502-048), B27 supplement (1×; Gibco, 12587-610), penicillin– streptomycin (1×), 2-mercaptoethanol (150 μM; e-Nacalai), bovine serum albumin (0.05%), KnockOut Serum Replacement (1%; Gibco), recombinant mouse basic fibroblast growth factor (50 ng/ml; Wako, 450-33), recombinant human activin A (20 ng/ml; R&D Systems, 338-AC), XAV939 (10 μM; Calbiochem), Y-27632 (5 μM; Wako), and heparin (1 μg/ml). ..

    Modification:

    Article Title: Chronic cerebral hypoperfusion induces venous dysfunction via EPAS1 regulation in mice.
    Article Snippet: To seed for endothelial differentiation, H9 were dissociated with accutase (Gibco, A1110501) into single cells, then plated onto Matrigel-coated plates at a density of 50,000 cells/cm2 in mTeSR1 medium supplemented with 5 μMROCK inhibitor, Y-27632 (STEMCELL Technologies, 72304). .. 24 h later, H9 cells were induced towards the mid-primitive streak differentiation pathway with 40 ng/ml recombinant human BMP-4 (R&D Systems, 314-BP/CF), 6 μM CHIR99021 (Sigma-Aldrich, SML1046), 4 μg/ml recombinant human FGF2 (R&D Systems, 233-FB/CF), PIK-90 (SigmaAldrich, 528117), recombinant human activin A (R&D Systems, 338- AC/CF) in Chemically Defined Medium (CDM) made up of 50% Ham’s F12 (Gibco, 31765092), 50% Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco, 31980097), 1mg/ml polyvinyl alcohol (SigmaAldrich, P8136), 1% chemically defined lipid concentrate (Gibco, 11905031), 450μM 1-thioglycerol (Sigma-Aldrich, M6145), 15μg/ml transferrin (Roche, 10652202001) and 0.7μg/ml recombinant human insulin (Roche, 11376497001). .. After 24 h, mid-primitive streak cells were differentiated into dorsal lateral mesoderm in CDM, supplemented with 40 ng/mL BMP4, 2.5μM GDC-0941 (STEMCELL Technologies, 73152), 10 μM Forskolin (Tocris, 1099), 2 μM SB505124 (Sigma-Aldrich, S4696), 100 ng/ml VEGF (R&D Systems, 293-VE/CF), 1 μM XAV939 (Tocris, 3748) and 200μg/mL L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich, A8960) Nature Communications | (2025) 16:6302 14 for 24 h. Following that, the dorsal lateral mesoderm was further differentiated towards either arterial endothelial induction or prevein induction, with a duration of 24 h. For arterial induction, CDM supplemented with 15 ng/mL recombinant human activin A, 250 nM DMH-1 (Tocris, 4126), 2.5 μM GDC-0941, 100ng/mL VEGF, 1 μM XAV939 and 200μg/ml L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate was used.

    Article Title: Chronic cerebral hypoperfusion induces venous dysfunction via EPAS1 regulation in mice
    Article Snippet: To seed for endothelial differentiation, H9 were dissociated with accutase (Gibco, A1110501) into single cells, then plated onto Matrigel-coated plates at a density of 50,000 cells/cm 2 in mTeSR1 medium supplemented with 5 μM ROCK inhibitor, Y-27632 (STEMCELL Technologies, 72304). .. 24 h later, H9 cells were induced towards the mid-primitive streak differentiation pathway with 40 ng/ml recombinant human BMP-4 (R&D Systems, 314-BP/CF), 6 μM CHIR99021 (Sigma-Aldrich, SML1046), 4 μg/ml recombinant human FGF2 (R&D Systems, 233-FB/CF), PIK-90 (Sigma-Aldrich, 528117), recombinant human activin A (R&D Systems, 338-AC/CF) in Chemically Defined Medium (CDM) made up of 50% Ham’s F12 (Gibco, 31765092), 50% Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco, 31980097), 1 mg/ml polyvinyl alcohol (Sigma-Aldrich, P8136), 1% chemically defined lipid concentrate (Gibco, 11905031), 450 μM 1-thioglycerol (Sigma-Aldrich, M6145), 15 μg/ml transferrin (Roche, 10652202001) and 0.7 μg/ml recombinant human insulin (Roche, 11376497001). .. After 24 h, mid-primitive streak cells were differentiated into dorsal lateral mesoderm in CDM, supplemented with 40 ng/mL BMP4, 2.5 μM GDC-0941 (STEMCELL Technologies, 73152), 10 μM Forskolin (Tocris, 1099), 2 μM SB505124 (Sigma-Aldrich, S4696), 100 ng/ml VEGF (R&D Systems, 293-VE/CF), 1 μM XAV939 (Tocris, 3748) and 200 μg/mL L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich, A8960) for 24 h. Following that, the dorsal lateral mesoderm was further differentiated towards either arterial endothelial induction or pre-vein induction, with a duration of 24 h. For arterial induction, CDM supplemented with 15 ng/mL recombinant human activin A, 250 nM DMH-1 (Tocris, 4126), 2.5 μM GDC-0941, 100 ng/mL VEGF, 1 μM XAV939 and 200 μg/ml L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate was used.

    Concentration Assay:

    Article Title: Method for producing pluripotent stem cells
    Article Snippet: On day 1 of the culture, medium exchange was performed with a MEF-conditioned medium containing bFGF at a final concentration of 20 ng/mL. (Step 2-A: Induction of Differentiation into Ectoderm Cells) For the cells prepared in Step 1 of Example 7, medium exchange was performed on day 2 of the culture with KnockOut-DMEM (Thermo Fisher Scientific K.K.) containing knockout serum replacement (Thermo Fisher Scientific K.K.) at a final concentration of 10%, Noggin (R&D Systems, Inc.) at a final concentration of 500 ng/mL, and SB431542 (Tocris Bioscience) at a final concentration of 10 μM. .. Medium exchange was performed using the same medium on day 3 of the culture, and thereby an ectoderm cell population could be obtained on day 4 of the culture. (Step 2-B: Induction of Differentiation into Mesoderm Cells) For the cells prepared in Step 1 of Example 7, medium exchange was performed on day 2 of the culture with an RPMI-B27 (Thermo Fisher Scientific K.K.) medium to which recombinant human Activin A (R&D Systems, Inc.) was added at a final concentration of 100 ng/mL. .. Medium exchange was performed on days 3, 4, and 5 of the culture with an RPMI-B27 (Thermo Fisher Scientific K.K.) medium to which recombinant human BMP4 (R&D Systems, Inc.) was added at a final concentration of 10 ng/mL, and thereby a mesoderm cell population could be obtained on day 6 of the culture. (Step 2-C: Induction of Differentiation into Endoderm Cells) For the cells prepared in Step 1 of Example 7, medium exchange was performed on day 2 of the culture with an MCDB-131 medium to which NaHCO3 at a final concentration of 2.5 g/L, Glutamax (Thermo Fisher Scientific K.K.) at a final concentration of 1%, glucose at a final concentration of 5.5 mM, FAF-BSA (Proliant Inc. and Lampire Biological Laboratories, Inc.) at a final concentration of 0.1%, ITS: X (Thermo Fisher Scientific K.K.) diluted 50000-fold, WNT3A (R&D Systems, Inc.) at a final concentration of 20 ng/mL, and Activin A at a final concentration of 100 ng/mL were added.

    Knock-Out:

    Article Title: Universal donor cells and related methods
    Article Snippet: .. : hESC aggregates; XF HA: DMEM/F12 containing GlutaMAX, supplemented with 10% v/v of Xeno-free KnockOut Serum Replacement, 1% v/v non-essential amino acids, 1% v/v penicillin/streptomycin (all from Life Technologies), 10 ng/mL heregulin-1β (Peprotech) and 10 ng/mL activin A (R&D Systems); SP: StemPro ® hESC SFM (Life Technologies); r0.2FBS: RPMI 1640 (Mediatech); 0.2% FBS (HyClone), 1x GlutaMAX-1 (Life Technologies), 1% v/v penicillin/streptomycin; ITS: Insulin-Transferrin-Selenium (Life Technologies) diluted 1:5000 or 1:1000; A100: 100 ng/mL recombinant human Activin A (R&D Systems); W50: 50 ng/mL recombinant mouse Wnt3A (R&D Systems); K25: 25 ng/mL recombinant human KGF (R&D Systems); IV: 2.5 μM TGF-β RI Kinase inhibitor IV (EMD Bioscience); db: DMEM HI Glucose (HyClone) supplemented with 0.5x B-27 Supplement (Life Technologies), 1x GlutaMAX, and 1% v/v penicillin/streptomycin; CTT3: 0.25 μM KAAD-Cyclopamine (Toronto Research Chemicals) and 3 nM TTNPB (Sigma-Aldrich); N50: 50 ng/mL recombinant human Noggin (R&D Systems); K50: 50 ng/mL recombinant human KGF (R&D Systems); E50: 50 ng/mL recombinant human EGF (R&D Systems). ..

    Article Title: Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin
    Article Snippet: .. CDM was prepared with Neurobasal medium (Gibco, 21103-049) and DMEM high glucose (Sigma, D6429), supplemented with N2 supplement (1×; Gibco, 17502-048), B27 supplement (1×; Gibco, 12587-610), penicillin– streptomycin (1×), 2-mercaptoethanol (150 μM; e-Nacalai), bovine serum albumin (0.05%), KnockOut Serum Replacement (1%; Gibco), recombinant mouse basic fibroblast growth factor (50 ng/ml; Wako, 450-33), recombinant human activin A (20 ng/ml; R&D Systems, 338-AC), XAV939 (10 μM; Calbiochem), Y-27632 (5 μM; Wako), and heparin (1 μg/ml). ..



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